Cells

Human embryonic stem (ES) cells

Assay and culture

  • As differentiation occurs embryoid bodies (EBs) are formed
  • LIF in the culture medium allows proliferation and maintenance of pluripotency
  • Removing LIF allows differentiation to occur
  • Hanging drop method enhances aggregation with less adhesion to the substrate
  • Days later disaggregate and grow on adhesive substrate
  • Use specific media supplements to cause differentiation into specific cell types

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