Cells
Human embryonic stem (ES) cells
Assay and culture
- As differentiation occurs embryoid bodies (EBs) are formed
- LIF in the culture medium allows proliferation and maintenance of pluripotency
- Removing LIF allows differentiation to occur
- Hanging drop method enhances aggregation with less adhesion to the substrate
- Days later disaggregate and grow on adhesive substrate
- Use specific media supplements to cause differentiation into specific cell types