Module 1932: The Cell

Studying cells 4: Data analysis

Standard curves

  • Assay in which you measure a series of known concentrations of e.g. protein alongside your unknown experimental sample
  • Determination requires standards for comparison, in order to quantify the results
  • The most common quantity measured in many assays is absorbance of light
  • By preparing samples with known amounts of protein and comparing their absorbances with that of the unknown, the unknown quantity can be estimated

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