MATS 10932: Cell Biology

Studying cells 2: Microscopy

Sample processing

Microscopy method Processing required
LM None
LM Fix, histological stain
LM Fix, embed in wax, section, histological stain
FM Fix with appropriate fixative, fluorochrome
FM Fix with appropriate fixative, primary antibody, secondary antibody
CM Fix with appropriate fixative, fluorochrome
CM Fix with appropriate fixative, primary antibody, secondary antibody
SEM Fix with appropriate fixative, dehydrate, dry, sputter coat with gold
TEM Fix with appropriate fixative, dehydrate, embed in resin, ultrathin sections, stain with electron dense stain

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