Bioreactors
Cell Distribution
- Plays a critical role in determining subsequent tissue formation
- High, even cell density=increased ECM production.
- Spinner flask - improved compared to static culture
- Convection of suspended cells into the scaffold leading to impacts between cells and scaffold resulting in cell adhesions.
- Intial cell density still higher at scaffold surfaces.
- Perfused systems - improved compared to spinner flask
- Convective flow of cell suspension directly through scaffold
- Higher viability and uniformity of cell distribution.