Bioreactors

Cell Distribution

  • Plays a critical role in determining subsequent tissue formation
  • High, even cell density=increased ECM production.
  • Spinner flask - improved compared to static culture
    • Convection of suspended cells into the scaffold leading to impacts between cells and scaffold resulting in cell adhesions.
    • Intial cell density still higher at scaffold surfaces.
  • Perfused systems - improved compared to spinner flask
    • Convective flow of cell suspension directly through scaffold
    • Higher viability and uniformity of cell distribution.

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