Tissue Engineering
Introduction
Module Summary:
- Introduction to tissue engineering
- The need for tissue engineering and current surgical solutions:Disease states, ageing, trauma, transplants, autografts, xenografts
- Cell sources: established cells, patient biopsies, stem cells, embryonic stem cells.
- Biomaterials for tissue replacement: review current materials used in tissue replacement
- Scaffolds for tissue engineering: development of appropriate scaffolds, scaffold properties, architecture, effects on cell responses, porosity
- Mimicking the extracellular matrix: peptides, growth factors, proteases
- Cell control via patterning and printing: controlling cell responses by patterned surfaces and printing specific molecules
- Hydrogels: synthetic and natural materials as hydrogels
- 3D generation of tissue: scaffold size limitations, regeneration of tissue, oxygen diffusion limitations, angiogenesis
- Specific examples of tissue engineered tissues: bone, cartilage, tendon/ligament, skin, liver, intervertebral disc
- Bioreactors: different methods for the culture of cells, rotary, flow perfusion
- Gene therapy and tissue engineering
- From concept to clinic: regulatory procedures, in vivo studies, patient trials, medical devices agency
Suggested reading:
- Principles of Tissue Engineering, Robert Lanza, Robert Langer, Joseph Vacanti. Academic Press, 2000.
- Biomaterials, medical devices, and tissue engineering : an integrated approach. Frederick H Silver. London : Chapman & Hall, 1994.
- Biomaterials Science, 1st Edition/2nd Edition, BD Ratner, AS Hoffman, FJ Schoen, Academic Press, 1996/2004.