Protein Mass Spectrometry Data Analysis

I am a bioinformatician employed by the University of Manchester to provide assistance with protein mass spectrometry experimental design and data analysis.

t: (+44/0) 161 529 4448
e: j.selley [at] manchester.ac.uk
a: B.2018a, Michael Smith Building
Biological Mass Spectrometry Core Research Facility
Faculty of Biology, Medicine, and Health
University of Manchester

  • Applying robust data analytical methodologies to mass spectrometry to facilitate researchers achieve high quality research particularly in the quantitation of proteins.
  • Ensuring the communication and provision of clear reporting of data and methodology.
  • Development of novel approaches to data analysis including the development of robust and easily understood statistical approaches to analysing mass spectrometry data.

  • Data management, processing, analysis, and reporting.
  • Software development (in multiple languages inc. C, C++, Java, Perl, Python, R).
  • Web resource development.
  • Relational database design and implementation.
  • Computer system administration / management (e.g., *NIX, Windows).
  • Problem solving.
  • Customer support and service provision to diverse client groups.
  • Project design and management.
  • Teaching and Training.

The Core Research Facilities are in part measured by their publication record, or the publications that they facilitate. If you use the BioMS CRF, we request that you would acknowledge the CRF in your publications. If you were unable to structure the experiment, or to complete the research conclusions without the expressed support of an individual in a CRF, then they should be included in the authors list (i.e., the staff member was heavily involved in the research).

If you are interested in reading further, you can find more information in publications in Nature such as this one (Gould, J. Core facilities: Shared support. Nature 519, 495–496 (2015). https://doi.org/10.1038/nj7544-495a).

To acknowledge the BioMS Core Research Facility, we ask that you use the Research Resource IDentification (RRID) resource. Our RRID number is SCR_020987.

Biography

I have worked in bioinformatics since 1995. Since 2003 I have worked in the field of proteomics, and worked for the (now) Biological Mass Spectrometry (BioMS) Core Research Facility (CRF) since 2006. I trained as a biochemist, with an interest in microbiology. In my role I have to be able to communicate complex scientific concepts to a wide range of audiences, to assist researchers design experiments using mass spectrometry, to be able to analyse complex data and assist in its interpretation, to design novel approaches to analysing data using software programming.

Expensive equipment is often purchased by the University (via various grants) so that researchers might share and use them. This equipment is placed into Core Research Facility laboratories to facilitate and improve research conducted by the University. Those CRFs are staffed by experts in maintaining and using the equipment, and interpreting the data those instruments produce.

The staff in CRFs are generally funded either from University finances, or from grants for specific projects. The University reclaims some of those finances by charging a fee for use of the Core Facilities. However, CRFs are not allowed to make a profit, and in fact inevitably run at a loss. The effectiveness of the CRFs are measured (in part) by their publication records - that is the publications they contribute to.

© Copyright 2023 Julian Selley. All Rights Reserved.

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